"BY 470/CM570 is a laboratory course designed to introduce and apply the basic principle of biochemistry and biotechnology (enzymology-enzyme activity, kinetic and inhibition, chromatography, gel electrophoresis, separation and purification of proteins). Biotechnology is a rapidly emerging field that uses a set of techniques to derive and explore the unique characteristics of living organisms and their components. Biotechnology is already commonly used in medicine, agriculture, environment to diagnose diseases, make high-yield pest-resistant crops, bioremediate contamination sites. The specific experiments in this laboratory are chosen to illustrate biotechnology principles thought in Biochemistry I and Biochemistry II.".
From Dr. Silvana Andreescu's introduction to the Biochemistry & Biotechnology laboratory class.
Additions introduced by Dr. Darie include Western blotting and a full proteomics experiment (SDS-PAGE, staining, band excision and digestion with trypsin, peptide extraction, LC-MS/MS run, data analysis and data interpretation.
Group 1
Independent project: Special gels for proteomics applications
Group 2
Independent project: Qualitative and quantitative analysis of protein contents in various protein shakes
Group 3
Independent project: Proteomic analysis of proteins from various types of vegetal and animal origins
Group 4
Independent project: Determination of alcohol content in various types of beer, wine and spirits
Group 5
Independent project: Beer: investigation of its protein patterns and characterization of its proteins by mass spectrometry
Group 6
Independent project: Yogurts: a mass spectrometry-based investigation
Group 7
Independent project: Caffeine and its determination in various beverages: an HPLC-based investigation
Teaching Assistant
Teaching Assistant
Pictures from the Lab
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